منابع مشابه
Plasma Membranes of the Rat Liver
A method is described for the rapid isolation of a plasma membrane fraction containing a high concentration of intact bile canaliculi from the rat liver. Isolated bile canaliculi retain most of the ultrastructural features exhibited in the intact liver cell. The final fraction contains 5'-nucleotidase activity at approximately the same concentration as that in previous preparations of plasma me...
متن کاملIsolation of Rat Liver Plasma Membranes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
متن کاملSpecific binding of calcitonin to rat liver plasma membranes.
The binding of human calcitonin (CT) was investigated in the plasma membrane fraction obtained from normal rat liver. The liver plasma membrane bound 125I-labeled [( 125I]) human CT, with increasing concentrations of the plasma membrane protein from 4.6-80 micrograms/ml, in a both time and temperature dependent manner. Specific binding of [125I] human CT was competitively inhibited by concentra...
متن کاملAlkaline ribonuclease and phosphodiesterase activity in rat liver plasma membranes.
The ribonuclease and phosphodiesterase activities of rat liver plasma membranes, purified from the crude nuclear fraction by centrifugation in an A-XII zonal rotor and flotation, were examined and compared. The plasma membrane is responsible for between 65 and 90% of the phosphodiesterase activity of the cell and between 25 and 30% of the particulate ribonuclease activity measured at pH8.7 in t...
متن کاملEnzymes of plasma membranes of liver.
The aim of the work was to provide tentative criteria for distinguishing between authentic and contaminating activities of plasma membrane preparations from rat liver. To this end, the specific activities and extractabilities were studied of eight activities found in the membrane preparations and in liver microsomes. Liver microsomes were examined since contaminants of the plasma membrane prepa...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1969
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.41.1.124